Scientific Researcher

To work within the group of Dr Ingo Greger at the MRC Laboratory of Molecular Biology (LMB), within a programme aimed at investigating AMPA glutamate receptor (AMPAR) operation and organisation at CA1 synapses. Specifically, to investigate the nano-positioning of AMPARs and their repositioning during LTP (long-term potentiation), using a combination of synaptic recordings (Penn et al., Neuron 2012; Watson et al., Elife 2017; Ho et al., J.Neurosci. Meth. 2020) and super-resolution imaging of hippocampal synapses (Watson et al., Nature Comms 2021).

The successful candidate will work in close collaboration with a PhD student and the LMB Light-microscopy team led by Dr. Nick Barry. We also closely collaborate with Cambridge University on studies of hippocampal synaptic plasticity mechanisms. In this project, we will characterise signalling and nano-positioning of synaptic/ dendritic glutamate receptors under baseline conditions and in response to LTP. LTP will be induced using various protocols including 2-photon glutamate uncaging onto individual dendritic spines. Towards this wider aim, we have established various state of the art approaches including 3D-STORM, STED and CRISPR/ Cas-9 genome editing. The successful candidate will have their own electrophysiology rig, and will have an opportunity to set-up a 2-photon rig (currently being purchased).

Main duties:

• To undertake research aimed at recording and imaging AMPARs at hippocampal synapses.
• To identify, develop and apply a broad range of techniques to pursue the research objectives.
• To present scientific work at seminars within the laboratory and at external meetings.
• To contribute to laboratory-wide discussions on developments within the field.
• Where appropriate, to draft scientific papers, and contribute to the overall preparation of research for publication.
• To contribute to the LMB’s mission in the public engagement of science, and the translation of research findings into improvements in health care.
• To assist in the training of PhD students and other members of the LMB.

Key responsibilities:

To plan your own work and objectives and manage your experimental work within the project along with your Supervisor.

To introduce and apply new techniques across a wide range of disciplines and to have the creativity and initiative to develop novel approaches and methods where required.

To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements.

To contribute to the smooth running of the group, including the effective use of resources, training of others and taking responsibility for use of communal facilities.

Working relationships:

You will report to Dr Ingo Greger and will interact and collaborate with other Postdoctoral Scientists, Research Support staff and students, not only in your group, but also across the LMB and with external groups as necessary.

Academic qualifications:

These should include a PhD in a relevant subject or due to complete PhD within 6 months.

Technical skills and expertise:

Experience of and ability to perform techniques relevant to the project.

Essential:
Experience with brain-slice electrophysiology.

Desirable:
Experience with super-resolution imaging and 2-photon imaging.

Track record of research:

This will include achievements, and contributions to published papers.

Other relevant evidence of:

• Commitment.
• Originality.
• Ability to communicate.
• Working with others.

Additional information:

This project will suit candidates who wish to further develop a hot research topic and who seek to work independently.